| WR | Garcon, Pierre | Pettis, Dante | ||
| WR | Goodwin, Marquise | Bourne, Kendrick | ||
| WR | Taylor, Trent | James, Richie | ||
| LT | Staley, Joe | |||
| LG | Tomlinson, Laken | Cooper, Jonathan | ||
| C | Richburg, Weston | Person, Mike | ||
| RG | Garnett, Josh | Magnuson, Erik | ||
| RT | McGlinchey, Mike | Gilliam, Garry | ||
| TE | Kittle, George | Celek, Garrett | Hikutini, Cole | |
| QB | Garoppolo, Jimmy | Beathard, CJ | ||
| RB | McKinnon, Jerick | Breida, Matt | Williams, Joe | Mostert, Raheem |
| FB | Juszcyk, Kyle | |||
| DE | Armstead, Arik | Blair, Ronald | Marsh, Cassius | |
| DT | Mitchell, Earl | Jones, DJ | ||
| DT | Buckner, DeForest | Day, Sheldon | ||
| DE | Thomas, Solomon | Attaochu, Jeremiah | ||
| SLB | Harold, Eli | Watson, Dekoda | ||
| MLB | Foster, Reuben* | Coyle, Brock | ||
| WLB | Smith, Malcolm | Warner, Fred | Toomer, Korey | |
| CB | Sherman, Richard | Williams, K’Waun | Reed, DJ | |
| SS | Tartt, Jaquiski | Harris, Marcell | ||
| FS | Colbert, Adrian | Powell, Tyvis | ||
| CB | Witherspoon, Ahkello | Ward, Jimmie | Moore, Tarvarius | |
| P | Pinion, Bradley | |||
| K | Gould, Robbie | |||
| LS | Nelson, Kyle | |||
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*Reuben Foster is suspender for 2 games
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